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Nitrocellulose Protein Microarray Slides

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Nitrocellulose microarray slides provide high protein-binding capacity and high-sensitivity detection for protein microarrays, antibody arrays, antigen arrays, biomarker research, and quantitative proteomics. ELEXAN Scientific supplies Grace Bio-Labs ONCYTE® nitrocellulose film slides, including AVID, NOVA, and SuperNOVA porous nitrocellulose substrates. Choose the appropriate substrate based on protein binding capacity, film thickness, detection method, spot morphology, and fluorescence dynamic range.

  AVID NOVA SuperNova Onyx
Film Type Porous Porous Porous Non-porous
Film Thickness 12 μm 7 μm 12 μm <200 nm
Dynamic Range 5-6 log 5-6 log 7+ log 4-5 log
Contact Angle 80° (most hydrophobic) 70° 60° 70°
Detection Method Colorimetric, Chemiluminescence,Fluorescence Fluorescence Fluorescence Fluorescence
Binding Capacity High High High Standard
Best for Detection flexibility · Antigen & antibody arrays · Colorimetric workflows Standard fluorescence arrays · Consistent sensitivity · High-density printing RPPA · Biomarker discovery · Wide concentration range samples Low-background fluorescence · Precise surface control · High-res scanning
Ideal User Labs needing multi-method flexibility Most protein microarray labs Quantitative proteomics researchers High-sensitivity fluorescence specialists

Nitrocellulose provides a three-dimensional porous surface with high protein-binding capacity. Compared with conventional flat glass substrates, porous nitrocellulose can provide substantially greater available surface area for protein immobilization, making it useful for high-sensitivity protein microarrays and multiplexed assays.

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